%0 Journal Article
%T Utilization of I百B每EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-百B Inhibitor Activity
%A Lin Yu-Ling
%A Chen Yen-Shun
%A Hsieh Jui-Hung
%A Lin Ching-Min
%J Biological Procedures Online
%D 2010
%I BioMed Central
%X NF-百B regulates several important expressions, such as cytokine release, anti-apoptosis, adhesion molecule expression, and cell cycle processing. Several NF-百B inhibitors have been discovered as an anti-tumor or anti-inflammatory drug. The activity of NF-百B transcription factor is negatively regulated by I百B binding. In this study, I百B assay system was established and I百B每EGFP fusion protein was used as an indicator to monitor the effects of substances on the I百B degradation. The results indicated that the chosen hydroquinone could inhibit the I百B degradation and cause the cell de-attachment from the bottom of culture plate. In addition, this system could also monitor the I百B degradation of microbial metabolite of natural mixtures of propolis. Thus, the I百B assay system may be a good system for drug discovery related to microbial metabolite.
%K Microbial metabolite
%K Antioxidant
%K I百B
%K EGFP
%K Hydroquinone
%K Propolis
%U http://www.biologicalproceduresonline.com/content/12/1/131