%0 Journal Article
%T Causticum hahnemanni, Conium maculatum and Lycopodium clavatum highly diluted medications decreases parasitemia in mice infected by Trypanosoma cruzi
%A Gislaine Janaina Sanchez Falkow Temporini
%A Carina Ribeiro Lopes
%A Camila Fernada Brustolin
%A Paula Fernanda Massini
%J International Journal of High Dilution Research
%D 2012
%I Universidade Estadual Paulista
%X Introduction: Benznidazole is the only medicine available in Brazil for Chagas ¡éa  a ¡é disease treatment, however it presents low efficacy in the chronic phase and several adverse effects [1-3]. Aim: Evaluate the effect of Causticum hahnemanni, Conium maculatum and Lycopodium clavatum [4-6] administered to mice infected with T. cruzi. Method: In blind randomized controlled trial 42 male Swiss mice, 8 weeks of age, have been grouped: GCaus  ¡éa  a? treated with C. hahnemanni 13cH (n=10), GCon  ¡éa  a? treated with C. maculatum 13 cH (n=11), GLy  ¡éa  a? treated with L. clavatum13cH (n=10) and CG  ¡éa  a?  control group (n=11) treated with 7% hydro alcoholic solution 13cH. The animals were infected intraperitoneally with 1.400 blood trypomastigotes of T. cruzi - Y strain. Medications were been prepared according to Brazilian Homeopathic Pharmacopoeia [7]. Medication was diluted in water (1mL/100mL) offered ad libitum, from amber recipient during 16 hours administered 48 hours before infection and 48, 96 and 144 hours after inoculation. Parasitological parameters assessed: total parasitemia (TP), maximum peak of parasites (MPP), pre-patent period (PPP) and area under curve (AUC). Parasitemia was evaluated daily counting from the first day of infection. Clinical parameters assessed: weight, temperature, water and food intake and excreta were measured counting from the 5   o day before infection until animals ¡éa  a ¡é death or checking negative parasitemia for 3 consecutive days. Mortality was registered for 75 days after infection. Ethics Committee for Experiments in Animals gave approval UEM 054/11. Statistical comparison of data was performed with Kruskal-Wallis test, with 5% significance. Results and Discussion: The diluted medications have been significantly reduced the parasitological parameters: MPP (p<0,0000), TP (p<0,0000) and AUC (9,7   ¡À3,5)x109; (6,2   ¡À1,7)x109; (5,5   ¡À1,7)x109; (5,7   ¡À1,5)x109 (p<0,0001) measured in trypomastigotes/mL, considering CG, GCaus, GCon and GLy respectively. Besides, they increased the PPP for GCaus, GCon and GLy (5,07   ¡À0,54; 5,40   ¡À1,29; 5,91   ¡À1,1) in relation to CG (4,75   ¡À1,00) (p<0,0001). Survival was significantly different between groups (p=0,0001), with Ly showing survival estimate of 0,29 (IC: 0,182  ¡éa  a?  1) versus 0,125 from CG (IC: 0,02  ¡éa  a?  0,782) until the 21st day of infection. GCaus and GCon showed survival estimate 0 for 18 and 17 days, respectively. GLy presented significant increase in water intake (p=0,0000) and higher temperature control, lowering hypothermia before death (p<0,0000). Concl
%K Chagas ¡éa  a ¡é disease
%K High dilutions
%K Mice
%K Trypanosoma cruzi
%U http://www.feg.unesp.br/~ojs/index.php/ijhdr/article/view/591