%0 Journal Article
%T Sensitization with atypical mycobacteria is a potent risk factor for cross-reaction with the delayed type hypersensitivity assay in mice
%A Eduardo Martins de Sousa
%A Fernando Bonfim de Bortoli
%A Bruna Daniella de Souza Silva
%A et al
%J Pathology and Laboratory Medicine International
%D 2010
%I Dove Medical Press
%X Eduardo Martins de Sousa1, Fernando Bonfim de Bortoli1, Bruna Daniella de Souza Silva1, Ediane Batista da Silva1, Scott M Irwin2, Ana Paula Junqueira Kipnis11Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goias, Goiania-Go, Brazil; 2Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, USAAbstract: Tuberculosis is a disease that infects approximately two billion people worldwide. The current diagnostic test utilizes purified protein derivative (PPD) obtained from Mycobacterium tuberculosis cultures to elicit a host delayed type hypersensitivity reaction to identify infected individuals. This reaction is manifested as an induration at 48 hours following intradermal i-njection. These experiments demonstrate that in a mouse model, repeated administrations of PPD do not elicit a positive tuberculin skin test (TST). However, prior sensitization to -environmental mycobacteria does induce a positive TST when administered via the intraperitoneal or oral route. This work has important implications regarding the specificity of the PPD reagent, disease diagnosis, and environmental mycobacteria.Keywords: tuberculin skin test, environmental mycobacteria, immune response
%U http://www.dovepress.com/sensitization-with-atypical-mycobacteria-is-a-potent-risk-factor-for-c-a4860