%0 Journal Article
%T Low-dose etoposide-treatment induces endoreplication and cell death accompanied by cytoskeletal alterations in A549 cells. Does the response involve senescence? The possible role of vimentin
%A Anna Litwiniec
%A Lidia Gackowska
%A Anna Helmin-Basa
%A Agnieszka £¿ury£¿
%A Alina Grzanka
%J Cancer Cell International
%D 2013
%I BioMed Central
%R 10.1186/1475-2867-13-9
%X After treatment with etoposide, selected biochemical and morphological parameters were examined, including: the activity of senescence-associated Ss-galactosidase, SAHF formation, cell cycle progression, the induction of p21Cip1/Waf1/Sdi1 and cyclin D1, DNA strand breaks, the disruption of cell membrane asymmetry/integrity and ultrastructural alterations. Vimentin and G-actin cytoskeleton was evaluated both cytometrically and microscopically.Etoposide induced a senescence-like phenotype in the population of A549 cells. Morphological alterations were nevertheless not directly coupled with other senescence markers including a stable cell cycle arrest, SAHF formation or p21Cip1/Waf1/Sdi1 induction. Instead, a polyploid, TUNEL-positive fraction of cells visibly grew in number. Also upregulation of cyclin D1 was observed. Here we present preliminary evidence, based on microscopic analyses, that suggest a possible role of vimentin in nuclear alterations accompanying polyploidization-depolyploidization events following genotoxic insults.
%U http://www.cancerci.com/content/13/1/9/abstract