%0 Journal Article
%T Influence of bushenhuoxue on primary visual cortex' BDNF damage in rat model of chronic elevated intraocular pressure
%A Xiang Li
%A Hong-Jian Guo
%A Xiao-Xia Wen
%A Zhao Xie
%J Guoji Yanke Zazhi
%D 2013
%I IJO Press
%R 10.3980/j.issn.1672-5123.2013.04.01
%X AIM: To observe the effect of traditional Chinese medicine(TCM)of bushenhuoxue on primary visual cortex(PVC)brain-derived neurotrophic factor(BDNF)in rat model of chronic elevated intraocular pressure(EIOP), and explore the mechanism of it initially. METHODS: The rat model of chronic EIOP was established by unilaterally cauterizing 3 episcleral veins, then 30 rats were divided into 3 groups randomly: control group, model group, and treatment group. After given drugs or normal saline for 8 weeks, the rats were put to death. The effect of intraocular pressure(IOP), expression of BDNF and ultrastructure of neuron cell in the PVC was observed. RESULTS: Unilaterally cauterizing episcleral veins increased IOP of the rat model obviously, there was significant difference compared with pre-operation(P<0.01). Semi-quantitative pathological analysis on PVC showed that BDNF of total area in the model group was(82438¡À2597.39)S/¦Ìm2,mean optical density was(1155.9¡À123.14), integrated optical density was(12915¡À673.28), compared with the control group {total area was(132370¡À7588.47)S/¦Ìm2, mean optical density was(5365¡À379.65), integrated optical density was(35102¡À2648.5)}, there were statistical differences(all P<0.05),there was statistical difference in BDNF of total area between model group and treatment group{(108980¡À9126.77)S/¦Ìm2, P<0.05}, significant difference in mean optical density between the model group and treatment group(3220.4¡À413.67, P<0.05), statistical difference in integrated optical density between the model group and treatment group(23821¡À3431.68, P<0.05). CONCLUSION: TCM of bushenhuoxue can repair the PVC damage in the rat model of chronic EIOP by enhancing expression of BDNF, improving ultrastructure of neuron cell.
%K glaucoma
%K rat model of chronic elevated intraocular pressure
%K TCM of bushenhuoxue
%K primary visual cortex
%K nissl bodies
%K ultrastructure of neuron cell
%U http://www.ies.net.cn/cn_publish/2013/4/201304001.pdf