%0 Journal Article
%T Isolation and Identification of Transforming Growth Factor ¦Â from In Vitro Matured Cumulus Oocyte Complexes
%A WIDJIATI
%A ARIEF BOEDIONO
%A SUTIMAN BAMBANG SUMITRO
%A AUCKY HINTING
%J HAYATI Journal of Biosciences
%D 2012
%I The Indonesian Biological Society & Bogor Agricultural University
%X Transforming growth factor-¦Â (TGF-¦Â) is a two-chain polypeptide with molecular weight of 25 kDa which takes significant role in the steroidogenesis process. In the ovarian oocyte in particular, TGF-¦Â has an important role in regulating reproductive function. TGF-¦Â represents a key intrafollicular protein that regulates follicle development and aromatization process. The purpose of this research was to characterize and identify a protein fraction of TGF-¦Â from the bovine isolated oocytes, which is synthesized during in vitro oocyte maturation process. Oocytes were collected from follicles with diameter of 3-8 mm. Oocytes were then matured in TCM 199 media supplemented with 5 ¦Ìg/mg LH, 3% BSA, and 50 ¦Ìg/ml gentamicin sulfate, and cultured in CO2 incubator (5%, 38.5 oC) for 20 hours. TGF-¦Â receptors were identified immunohistochemically. Characteristics of the TGF-¦Â protein were determined using SDS PAGE and TGF-¦Â specification was tested using Western Blotting. The results showed that TGF-¦Â receptors were identified and found in cumulus oocyte complexes (COCs). TGF-¦Â protein was isolated from bovine oocytes with molecular weight 25 kDa and it was identified by Western blotting methods in the same molecular weight.
%K transforming growth factor-¦Â (TGF-¦Â)
%K cumulus oocyte complexes (COCs)
%K identification
%U http://journal.ipb.ac.id/index.php/hayati/article/view/4818/3272