%0 Journal Article
%T Wound trauma alters ionizing radiation dose assessment
%A Juliann G Kiang
%A Bradley R Garrison
%A True M Burns
%A Min Zhai
%A Ian C Dews
%A Patrick H Ney
%A Lynnette H Cary
%A Risaku Fukumoto
%A Thomas B Elliott
%A G Ledney
%J Cell & Bioscience
%D 2012
%I BioMed Central
%R 10.1186/2045-3701-2-20
%X In this report, such confounding effects were addressed. Mice were given 60Co ¦Ã-photon radiation followed by skin wounding. Wound trauma exacerbated radiation-induced mortality, body-weight loss, and wound healing. Analyses of DNA damage in bone-marrow cells and peripheral blood mononuclear cells (PBMCs), changes in hematology and cytokine profiles, and fundamental clinical signs were evaluated. Early biomarkers (1 d after RCI) vs. irradiation alone included significant decreases in survivin expression in bone marrow cells, enhanced increases in ¦Ã-H2AX formation in Lin+ bone marrow cells, enhanced increases in IL-1¦Â, IL-6, IL-8, and G-CSF concentrations in blood, and concomitant decreases in ¦Ã-H2AX formation in PBMCs and decreases in numbers of splenocytes, lymphocytes, and neutrophils. Intermediate biomarkers (7 ¨C 10 d after RCI) included continuously decreased ¦Ã-H2AX formation in PBMC and enhanced increases in IL-1¦Â, IL-6, IL-8, and G-CSF concentrations in blood. The clinical signs evaluated after RCI were increased water consumption, decreased body weight, and decreased wound healing rate and survival rate. Late clinical signs (30 d after RCI) included poor survival and wound healing.Results suggest that confounding factors such as wounding alters ionizing radiation dose assessment and agents inhibiting these responses may prove therapeutic for radiation combined injury and reduce related mortality.Radiation injury combined with other injuries were observed at Hiroshima and Nagasaki, Japan, where approximately 60% of victims received radiation alone with approximately 40% of victims having other injuries concurrent with radiation injury [1,2]. After the Chernobyl reactor meltdown, 10% of 237 victims exposed to radiation received thermal burns [3]. In animals including mice [4,5], rats [6,7], guinea pigs [8], dogs [9], and swine [10,11], burns and wounds usually increase mortality after otherwise non-lethal irradiation. In mice, irradiation combined with wounds [4
%K Radiation
%K Wound
%K Combined injury
%K Lymphocyte
%K Neutrophil
%K Platelet
%K Splenocyte
%K ¦Ã-H2AX
%K Cytokine
%K DNA damage
%K Survivin
%U http://www.cellandbioscience.com/content/2/1/20