%0 Journal Article
%T Functional and genetic interactions of TOR in the budding yeast Saccharomyces cerevisiae with myosin type II-deficiency (myo1忖)
%A Glorivee Pag芍n-Mercado
%A Ednalise Santiago-Cartagena
%A Pearl Akamine
%A Jos谷 R Rodr赤guez-Medina
%J BMC Cell Biology
%D 2012
%I BioMed Central
%R 10.1186/1471-2121-13-13
%X Here we proved that TORC1 signaling was down regulated in the myo1忖 strain. While a tor1忖 mutant strain had increased viability relative to myo1忖, a combined myo1忖tor1忖 mutant strain showed significantly reduced cell viability. Synthetic rescue of the tor2-21ts lethal phenotype was observed in the myo1忖 strain in contrast to the chs2忖 strain, a chitin synthase II null mutant that also activates the PKC1 CWIP and exhibits cytokinesis defects very similar to myo1忖, where the rescue effect was not observed. We observed two pools of Slt2p, the final Mitogen Activated Protein Kinase (MAPK) of the PKC1 CWIP; one pool that is up regulated by heat shock and one that is up regulated by the myo1忖 stress. The cell wall stress sensor WSC1 that activates PKC1 CWIP under other stress conditions was shown to act as a negative regulator of TORC1 in the myo1忖 mutant. Finally, the repression of TORC1 was inversely correlated with the activation of PKC1 in the myo1忖 strain.Regulated expression of TOR1 was important in the activation of the PKC1 CWIP in a myo1忖 strain and hence its survival. We found evidence that the PKC1 and TORC1 pathways share a common upstream regulator associated with the cell wall stress sensor WSC1. Surprisingly, essential TORC2 functions were not required in the myo1忖 strain. By understanding how yeast mounts a concerted stress response, one can further design pharmacological cocktails to undermine their ability to adapt and to survive.
%K PKC1
%K SLT2/MPK1
%K WSC1
%K Tor2-21
%K Fungal cell wall
%U http://www.biomedcentral.com/1471-2121/13/13/abstract