%0 Journal Article
%T ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTION
%A ALEXANDRA BOLEMAN
%A ADA CEAN
%A V. CARAB£¿
%A N. P£¿CAL£¿
%J Lucrari Stiintifice : Zootehnie si Biotehnologii
%D 2009
%I Facultatea de Zootehnie si Biotehnologii, Timisoara
%X In order to evaluate the esterasic activity within the viable embryos we used the Fluorescein diacetate(FDA) staining test. For staining was used a 0.5 mg/ml FDA stock solution. The embryos were recoveredat 48 hours post coitus from superovulated Swiss mouse females. Before staining the embryos weremicroscopically evaluated by morphological criteria and classified in 4 quality codes. The two methodsused for quality and viability assessment were correlated applying Pearson coefficient. The calculatedvalue of the Pearson coefficient (r=1) showed a strong correlation between the two methods used andindicate FDA staining test and esterasic activity as a fast, easy and reliable method for embryo viabilityassessment.
%K embryo
%K viability
%K FDA
%K esterasic activity
%U http://usab-tm.ro/fileadmin/fzb/simpozion%202009/Volumul1/biotehnologii/Boleman.pdf