%0 Journal Article
%T Up-regulation of COX-2/PGE2 by endothelin-1 via MAPK-dependent NF-百B pathway in mouse brain microvascular endothelial cells
%A Chih-Chung Lin
%A Hsi-Lung Hsieh
%A Ruey-Horng Shih
%A Pei-Ling Chi
%A Shin-Ei Cheng
%A Chuen-Mao Yang
%J Cell Communication and Signaling
%D 2013
%I BioMed Central
%R 10.1186/1478-811x-11-8
%X The data obtained with Western blotting, RT-PCR, and immunofluorescent staining analyses showed that ET-1-induced COX-2 expression was mediated through an ETB-dependent transcriptional activation. Engagement of Gi- and Gq-protein-coupled ETB receptors by ET-1 led to phosphorylation of ERK1/2, p38 MAPK, and JNK1/2 and then activated transcription factor NF-百B. Moreover, the data of chromatin immunoprecipitation (ChIP) and promoter reporter assay demonstrated that the activated NF-百B was translocated into nucleus and bound to its corresponding binding sites in COX-2 promoter, thereby turning on COX-2 gene transcription. Finally, up-regulation of COX-2 by ET-1 promoted PGE2 release in these cells.These results suggested that in mouse bEnd.3 cells, activation of NF-百B by ETB-dependent MAPK cascades is essential for ET-1-induced up-regulation of COX-2/PGE2 system. Understanding the mechanisms of COX-2 expression and PGE2 release regulated by ET-1/ETB system on brain microvascular endothelial cells may provide rationally therapeutic interventions for brain injury or inflammatory diseases.Cerebral capillary and microvascular endothelial cells play an active role in maintaining cerebral blood flow, microvascular tone and blood-brain barrier (BBB) functions [1]. In the development of various vascular diseases, an early finding is dysfunction of the vascular endothelium that is closely related to clinical events in patients with atherosclerosis and hypertension [2,3]. The vasoactive mediators such as endothelin (ET) could be produced by endothelial cells to maintain hemodynamic responses. Production and release of ETs from cultured endothelial cells are regulated at transcription and translation levels by a variety of chemical and physical stimuli and the levels of ET, ET-1 especially, are elevated in shock, myocardial infarction, and kidney failure indicative of enhanced formation in these diseases [4]. Moreover, the bioactivity of ET-1 triggers vasoconstriction and pro-infl
%K Endothelin-1
%K COX-2
%K MAPK
%K NF-百B
%K Brain microvascular endothelial cells
%U http://www.biosignaling.com/content/11/1/8