%0 Journal Article
%T Immunophenotypic enumeration of CD4+ T-lymphocyte values in human immunodeficiency virus-seronegative adults in Eastern India
%A Dash M
%A Padhi S
%A Panda P
%A Parida B
%J International Journal of Medicine and Biomedical Research
%D 2012
%I Michael Joanna Publications
%X Background: The enumeration of CD4+ T-lymphocytes in Human Immunodeficiency Virus (HIV)-infected individuals is an essential tool for staging HIV disease, to make decisions for initiation of anti-retroviral therapy (ART), for monitoring response to ART and to initiate chemoprophylaxis against opportunistic infections. Therefore, it is important to know the level of immunocompetence of a particular geographical region by enumerating the baseline CD4+ T-lymphocytes in HIV-seronegative healthy adults. Aim: The aim was to enumerate CD4+ T-lymphocytes counts of healthy HIV-seronegative adults in eastern India. Materials and Methods: Blood samples were obtained from hundred HIV-seronegative healthy adults (mean age 32.6¡À11.3 years) who attended integrated counselling and testing centre (ICTC-1) for HIV information. Immunophenotypic enumeration of CD4+ T-lymphocytes was carried out using flow cytometer. Results: The mean absolute CD4+ T-lymphocytes count was 823.9(¡À243.4)cells/¦Ìl. The established range of CD4+ T-lymphocyte counts for men and women were 338 ¨C 1292 cells/¦Ìl (mean 793.4¡À243.5 cells/¦Ìl) and 402 ¨C 1321 cells/¦Ìl (mean 885.9¡À234.8 cells/¦Ìl) respectively. Women had significantly higher absolute CD4+ T-lymphocyte counts (p<0.001) when ompared to men. The distribution of mean absolute CD4+ T-lymphocyte counts among different age groups showed that individuals within 18 to 27 years of age group had significantly higher CD4+ T-lymphocyte counts of 893.3¡À43.4) cells/¦Ìl. Conclusions: Our findings of CD4+ T-lymphocyte counts among HIV-seronegative adults in east India adds to the emerging data supporting the presence of significant differences in reference to CD4+ T-lymphocyte counts between different populations within and outside the country.
%K CD4+ T-lymphocytes
%K HIV
%K ART
%K immunocompetence
%U http://www.ijmbr.com/reviewed/1.3.12.pdf