%0 Journal Article
%T Rab11-FIP3 is a cell cycle-regulated phosphoprotein
%A Louise L Collins
%A Glenn Simon
%A Johanne Matheson
%A Christine Wu
%A M Clare Miller
%A Tetsuhisa Otani
%A Xinzi Yu
%A Shigeo Hayashi
%A Rytis Prekeris
%A Gwyn W Gould
%J BMC Cell Biology
%D 2012
%I BioMed Central
%R 10.1186/1471-2121-13-4
%X We identify four sites of phosphorylation of FIP3 in vivo, S-102, S-280, S-347 and S-450 and identify S-102 as a target for Cdk1-cyclin B in vitro. Of these, we show that S-102 is phosphorylated in metaphase and is dephosphorylated as cells enter telophase. Over-expression of FIP3-S102D increased the frequency of binucleate cells consistent with a role for this phospho-acceptor site in cytokinesis. Mutation of S-280, S-347 or S-450 or other previously identified phospho-acceptor sites (S-488, S-538, S-647 and S-648) was without effect on binucleate cell formation and did not modulate the distribution of FIP3 during the cell cycle. In an attempt to identify a functional role for FIP3 phosphorylation, we report that the change in FIP3 distribution from cytosolic to membrane-associated observed during progression from anaphase to telophase is accompanied by a concomitant dephosphorylation of FIP3. However, the phospho-acceptor sites identified here did not control this change in distribution.Our data thus identify FIP3 as a cell cycle regulated phosphoprotein and suggest dephosphorylation of FIP3 accompanies its translocation from the cytosol to membranes during telophase. S102 is dephosphorylated during telophase; mutation of S102 exerts a modest effect on cytokinesis. Finally, we show that de/phosphorylation of the phospho-acceptor sites identified here (S-102, S-280, S-347 and S-450) is not required for the spatial control of recycling endosome distribution or function.Membrane traffic to the furrow is an essential facet of cytokinesis [1-5]. The plasma membrane of the furrow of dividing cells has a distinct lipid and protein composition as compared to the rest of the plasma membrane, with the furrow enriched in cholesterol and phosphatidylinositol 4, 5-bisphosphate (for example) and various proteins involved in intracellular membrane trafficking (e.g. dynamin, SNAREs, etc.) [2,5,6]. Exocytosis of intracellular membranes occurs to the furrow and intercellular bridge
%K Cytokinesis
%K Rab11-FIP3
%K Cdk1
%K Endosomes
%U http://www.biomedcentral.com/1471-2121/13/4