%0 Journal Article
%T Cytotoxicity, anti-angiogenic, apoptotic effects and transcript profiling of a naturally occurring naphthyl butenone, guieranone A
%A Victor Kuete
%A Tolga Eichhorn
%A Benjamin Wiench
%A Benjamin Krusche
%A Thomas Efferth
%J Cell Division
%D 2012
%I BioMed Central
%R 10.1186/1747-1028-7-16
%X The results indicated that GA was active on 91.67% of the 12 tested cancer cell lines, the IC50 values below 4 ¦Ìg/ml being recorded on 83.33% of them. In addition, the IC50 values obtained on human lymphoblastic leukemia CCRF-CEM (0.73 ¦Ìg/ml) and its resistant subline CEM/ADR5000 (1.01 ¦Ìg/ml) and on lung adenocarcinoma A549 (0.72 ¦Ìg/ml) cell lines were closer or lower than that of doxorubicin. Interestingly, low cytotoxicity to normal hepatocyte, AML12 cell line was observed. GA showed anti-angiogenic activity with up to 51.9% inhibition of the growth of blood capillaries on the chorioallantoic membrane of quail embryo. Its also induced apotosis and cell cycle arrest. Ingenuity Pathway Analysis identified several pathways in CCRF-CEM cells and functional group of genes regulated upon GA treatment (P < 0.05), the Cell Cycle: G2/M DNA Damage Checkpoint Regulation and ATM Signaling pathways being amongst the four most involved functional groups.The overall results of this work provide evidence of the cytotoxic potential of GA and supportive data for its possible use in cancer chemotherapy.Malignant diseases are responsible of approximately 13% of all deaths each year in the world. About 12.7 million cancer cases and 7.6 million cancer deaths are estimated to have occurred in 2008; of these, 56% of the cases and 64% of the deaths occurred in the economically developing world [1]. Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer death among females, accounting for 23% of the total cancer cases and 14% of the cancer deaths; Lung cancer is the leading cancer site in males, comprising 17% of the total new cancer cases and 23% of the total cancer deaths [1]. It is reported that cancer is the leading cause of death in the developed world and the second leading cause of death in the developing world [1]. In 2000, leukemia represented about 3% of the almost seven million deaths due to cancer that year, and about 0.35% of all deaths from any
%K Angiogensis
%K Apoptosis
%K Cytotoxicity
%K Guieranone A
%K Microarray
%K Pharmacogenomics
%U http://www.celldiv.com/content/7/1/16