%0 Journal Article
%T Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles
%A Jane de Lartigue
%A Ingrid Brust-Mascher
%A Jonathan M Scholey
%J Cell Division
%D 2011
%I BioMed Central
%R 10.1186/1747-1028-6-8
%X The basic mechanism, involving an inverse correlation between poleward flux and spindle elongation is qualitatively similar in these systems, but quantitative differences exist. In S2 cells, poleward flux is only partially suppressed and the rate of anaphase B spindle elongation increases with the extent of suppression. Also, EB1-labelled microtubule plus ends redistribute away from the poles and towards the interpolar microtubule overlap zone, but this is less pronounced in S2 cells than in embryos. Finally, as in embryos, tubulin FRAP experiments revealed a reduction in the percentage recovery after photobleaching at regions proximal to the pole.The basic features of the anaphase B switch, involving the suppression of poleward flux and reorganization of growing microtubule plus ends, is conserved in these systems. Thus S2 cells may be useful for rapidly identifying novel components of this switch. The quantitative differences likely reflect the adaptation of embryonic spindles for rapid, streamlined mitoses.Mitosis is mediated by the mitotic spindle, a cellular machine composed of microtubules (MTs) and mitotic motors [1-5]. The critical function of mitosis is revealed at its climax, during anaphase, at which point the spindle coordinates separation of the sister chromatids to opposite spindle poles (anaphase A) and spindle elongation (anaphase B) in preparation for cytokinesis [6-8]. In the Drosophila melanogaster syncytial blastoderm stage embryo, highly dynamic MTs drive remarkably rapid movements of the chromosomes and spindle poles. Anaphase B spindle elongation is proposed to depend on an interpolar (ip) MT sliding filament mechanism generated by homotetrameric kinesin-5 motors and an "on-off" switch orchestrated by the suppression of poleward MT flux [9-13]. The current model of the mechanism underlying this anaphase B "switch" postulates that the pre-anaphase B spindle is maintained at a steady state length by the balance between ipMT sliding and ipMT depo
%U http://www.celldiv.com/content/6/1/8