%0 Journal Article
%T Rapid determination of natural steroidal hormones in saliva for the clinical diagnoses
%A Jin-Aa Oh
%A Ho-Sang Shin
%J Chemistry Central Journal
%D 2012
%I BioMed Central
%R 10.1186/1752-153x-6-22
%X A gas chromatography-mass spectrometric assay was developed for rapid simultaneous determination of E1, E2, E3, P and T in saliva for clinical diagnoses. Extraction was achieved with a liquid extraction using 3.0 mL of pentane. The extract was dried and silylated with N-methyl-N-(trimethylsilyl) trifluoroacetamide/NH4I (100:2) under a catalysis of 1.5% dithioerythritol for 10 min at 90¡ãC. The accuracy of the analytes was in the range of 96% to 112% at concentrations of 0.05 and 0.10 ¦Ìg/L (5.0 and 10.0 ¦Ìg/L for E3), respectively, with relative standard deviations of less than 11%. The lowest quantification limits were from 0.002 to 0.6 ¦Ìg/L for 1.0 mL of saliva.Natural steroidal hormones were detected in the concentration ranges of nd to 0.2 ¦Ìg/L in human saliva. The salivary testosterone values in the patients with prostatic carcinoma were significantly lower than in normal males. The method may useful in numerous clinical and health-related diagnoses.Saliva samples are easily collectable, non-invasive, and pose no risk to the health of the subjects. Salivary concentrations of steroids generally reflect serum concentrations of free active steroids; a significant correlation between salivary and serum steroid concentrations was shown in men [1-4]. Therefore, saliva has been used in a number of human and nonhuman studies to determine the presence of a wide range of substances, including biological substances and pharmaceutical drugs [5]. The monitoring of natural steroidal hormones, such as estrone (E1), 17¦Â-estradiol (E2), estriol (E3), progesterone (P), and testosterone (T), in saliva has attracted much attention due to its numerous potential clinical and health-related applications [1-4,6-14].Clinical monitoring of E2 is desirable for the study and treatment of hormone-dependent carcinomas [5], and investigating ovarian function [6]. The measurement of salivary E3 concentrations could be useful in evaluating antidepressant use during pregnancy [7] and the simultane
%K Clinical diagnosis
%K Natural steroidal hormones
%K Saliva
%K GC-MS
%U http://journal.chemistrycentral.com/content/6/1/22