%0 Journal Article
%T Reevaluating the Concept of Treating Experimental Tumors with a Mixed Bacterial Vaccine: Coley¡¯s Toxin
%A C. Maletzki
%A U. Klier
%A W. Obst
%A B. Kreikemeyer
%A M. Linnebacher
%J Clinical and Developmental Immunology
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/230625
%X Several decades after Coley’s initial work, we here systematically analyzed tumoricidal as well as immunostimulatory effects of the historical preparation Coley’s Toxin (CT), a safe vaccine made of heat-inactivated S. pyogenes and S. marcescens. First, by performing in vitro analysis, established human pancreatic carcinoma cell lines responded with dose- and time-dependent growth inhibition. Effects were attributed to necrotic as well as apoptotic cell death as determined by increased Caspase 3/7 levels, raised numbers of cells with sub-G1-DNA, and induced p expression, indicative for cell cycle arrest. Besides, CT effectively stimulated human peripheral blood leukocytes (huPBL) from healthy volunteers. Quantitative gene expression analysis revealed upregulated mRNA levels of selected Toll-like receptors. Flow cytometric phenotyping of CT-stimulated huPBLs identified raised numbers of CD25
%U http://www.hindawi.com/journals/cdi/2012/230625/