%0 Journal Article
%T Efectos del 芍cido f車lico sobre algunas variables morfom谷tricas del timo de ratas adolescentes con s赤ndrome fetal alcoh車lco Effects of folic acid on some morphometric variables of the thymus of adolescent rats with fetal alcohol syndrome
%A Aleida Herrera Batista
%A Melvis Tayl赤n Zumeta Dub谷
%A Maritza Gonz芍lez Bravo
%J Revista Cubana de Investigaciones Biomˋˋdicas
%D 2012
%I ECIMED
%X Se pretende determinar los efectos protectores sobre el timo de ratas adolescente con s赤ndrome fetal alcoh車lico experimental, para lo cual se utilizaron 24 ratas Wistar adolescentes y gestantes divididas en 4 grupos: A) tratadas con etanol, B) tratadas con etanol y 芍cido f車lico, C) tratadas solo con 芍cido f車lico y D) grupo no tratado. El tratamiento se extendi車 durante toda la gestaci車n hasta el parto. Se tomaron 2 cr赤as por cada madre. A las cr赤as se les practic車 la eutanasia a los 35 d赤as de vida posnatal. Se extrajo el timo y se obtuvieron cortes de 4 micr車metros y se colorearon con H/E. Se utiliz車 el programa digital para morfometr赤a Image Tool. Se midieron las 芍reas de la corteza y m谷dula del timo, as赤 como la relaci車n entre ambas variables. Se realiz車 ANOVA de 2 v赤as. El 芍rea de la corteza del timo fue significativamente menor en el grupo tratado con etanol en relaci車n con el resto de los grupos. Los hijos de las madres tratadas con 芍cido f車lico mostraron los valores mayores en el 芍rea de la corteza, y el grupo tratado con etanol y 芍cido f車lico mostr車 un patr車n muy similar al control. Se concluy車 que el etanol provoca cambios histopatol車gicos evaluados como atrofia de la corteza del timo y que el 芍cido f車lico suministrado a la madre durante la gestaci車n tiene efecto protector sobre esta anomal赤a. The study is intended to determine the protective effects of folic acid on the thymus of adolescent rats with experimental fetal alcohol syndrome. To this end, 24 adolescent and pregnant Wistar rats were used, which were divided in 4 groups: A) treated with ethanol, B) treated with ethanol and folic acid, C) treated only with folic acid, and D) not treated. Treatment extended throughout the entire gestation period until delivery. Two offspring were chosen from each mother. The offspring were euthanized at 35 days of postnatal life. The thymus was extracted and 4-micrometer cutouts were obtained, which were colored with H/E. The digital program Image Tool was used for morphometry. Measurements were taken of areas of the thymic cortex and medulla, and the relationship between the two variables was determined. A two-way ANOVA was performed. The thymic cortex area was significantly smaller in the group treated with ethanol than in the other groups. Offspring of mothers treated with folic acid showed the highest cortex area values, and the group treated with ethanol and folic acid exhibited a pattern very similar to the control group. It was concluded that ethanol brings about histopathological changes evaluated as thymic cortex atrophy, and that the folic aci
%K s赤ndrome alcoh車lico fetal
%K morfometr赤a
%K timo
%K alcoholismo
%K adolescencia
%K fetal alcohol syndrome
%K morphometry
%K thymus
%K alcoholism
%K adolescence
%U http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-03002012000100007