%0 Journal Article
%T A HPLC-DAD method for the simultaneous determination of five marker components in the traditional herbal medicine Bangpungtongsung-san
%A Weon Jin
%A Yang Hye
%A Ma Jin
%A Ma Choong
%J Pharmacognosy Magazine
%D 2011
%I Medknow Publications
%X Background: Bangpungtongsung-san, one of the traditional herbal medicines, was known to be a prescription for obesity. Objective: For the simultaneous determination of five components (paeoniflorin, 6-gingerol, decursin, geniposide, and glycyrrhizin) in Bangpungtongsung-san, a high-performance liquid chromatography with diode-array detector method was established. Materials and Methods: To develop the method, a reverse phase column, DIONEX C 18 (5 ¦Ìm, 120  , 4.6 mm ¡Á 150 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 230, 240, and 254 nm. Method validation was accomplished by linearity, precision test, and recovery test. Results: All calibration curves of components showed good linearity (R 2 > 0.9959). The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 0.17 ¦Ìg/ml and 0.04 to 0.53 ¦Ìg/ml, respectively. The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%. In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%. Conclusion: This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.
%K Bangpungtongsung-san
%K diode-array detector
%K high-performance liquid chromatography
%K marker component
%K validation
%U http://www.phcog.com/article.asp?issn=0973-1296;year=2011;volume=7;issue=25;spage=60;epage=64;aulast=Weon