%0 Journal Article
%T Screening of molluscicidal strain against Oncomelania hupensis from the rhizosphere of medicinal plant Phytolacca acinosa Roxb
%A Guo Danzhao
%A Chen Jun
%A Du Xiangping
%A Han Bangxing
%J Pharmacognosy Magazine
%D 2010
%I Medknow Publications
%X The research aimed to screen and exploit molluscicidal microorganisms against Oncomelania hupensis, from the rhizosphere of medicinal plant, Phytolacca acinosa Roxb., and one strain named as SL-30 was obtained with excellent molluscicidal activity. The freeze-dried powder of exocellular broth (EXB) of SL-30 could kill 100% of snails at a concentration of 48 mg/l for a submerged period of 24 h, with stabile molluscicidal activity at a temperature lower than 60¡ãC; furthermore, it could be gradually degraded after exposure to illumination for 15 days. The freeze-dried powder of SL-30¡äs EXB was safe to fresh fish and shrimp, even at a concentration beyond LC 90 of 24-h exposure period. The glycogen and total protein content of soft tissues of snails decreased after treating with SL-30¡äs EXB, and glycogen content¡äs decreasing rate had a linear relationship with molluscicidal activity. Finally, phylogenetic analysis based on ITS sequence showed that strain SL-30 had a higher similarity to Aspergillus fumigatus with bootstrap value 98%; accordingly, it was identified as a species of Aspergillus.
%K ITS sequence
%K molluscicidal activity
%K Oncomelania hupensis
%K Phytolacca acinosa Roxb.
%K rhizosphere
%K strain SL-30
%U http://www.phcog.com/article.asp?issn=0973-1296;year=2010;volume=6;issue=23;spage=159;epage=165;aulast=Guo