%0 Journal Article
%T Biological Fingerprinting of Herbal Samples by Means of Liquid Chromatography
%A £¿ukasz Cie£¿la
%J Chromatography Research International
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/532418
%X Biological chromatographic fingerprinting is a relatively new concept in the quality control of herbal samples. Originally it has been developed with the application of HPLC, and recently herbal samples' biological profiles have been obtained by means of thin-layer chromatography (TLC). This paper summarizes the application of liquid chromatographic techniques for the purpose of biological fingerprint analysis (BFA) of complex herbal samples. In case of biological TLC fingerprint, which is a relatively novel solution, perspectives of its further development are outlined in more detail. Apart from already published data, some novel results are also shown and briefly discussed. The paper aims at drawing scientists' attention to the unique solutions offered by biological fingerprint construction. 1. Introduction Fingerprint construction has become an important quality control tool of herbal samples in the light of constantly growing interest in natural origin medicines. Fingerprint analysis has been accepted by WHO as a methodology for the quality control of herbal samples [1, 2]. It is applied to identify closely related plant species, to detect adulterations, to control the extraction process or to study the quality of a finished product. Herbal sample fingerprint can be defined as a set of characteristic chromatographic or spectroscopic signals, whose comparison leads to an unambiguous sample recognition. Several chromatographic methods have been applied for fingerprint construction, namely, high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), gas chromatography (GC), or high-speed counter current chromatography (HSCCC). However, it is difficult to indicate with 100% certainty which signals (peaks, bands, etc.) should be present in the obtained fingerprint to confirm sample identity. For that purpose the analyzed sample can be compared with a defined Botanical Reference Material (BRM) or a set of standard compounds. Defining and obtaining BRM for every plant species is a difficult task, therefore, new solutions are sought for fingerprint comparison. More recently a concept of multiple fingerprints construction and multidimensional fingerprinting have gained much attention, as large amount of chromatographic and/or spectroscopic signals enable more comprehensive data analysis [3]. Multiple chromatographic fingerprint consists of more than one chromatographic profile [3], while in multidimensional fingerprinting hyphenated detectors are used (e.g., DAD and MS) that both record eluting compounds [1]. Usually the set of obtained
%U http://www.hindawi.com/journals/cri/2012/532418/