%0 Journal Article
%T Effect of nicotine on exocytotic pancreatic secretory response: role of calcium signaling
%A Chowdhury Parimal
%A Udupa Kodetthoor B
%J Tobacco Induced Diseases
%D 2013
%I BioMed Central
%R 10.1186/1617-9625-11-1
%X Background Nicotine is a risk factor for pancreatitis resulting in loss of pancreatic enzyme secretion. The aim of this study was to evaluate the mechanisms of nicotine-induced secretory response measured in primary pancreatic acinar cells isolated from Male Sprague Dawley rats. The study examines the role of calcium signaling in the mechanism of the enhanced secretory response observed with nicotine exposure. Methods Isolated and purified pancreatic acinar cells were subjected to a nicotine exposure at a dose of 100 ĻĖM for 6 minutes and then stimulated with cholecystokinin (CCK) for 30 min. The cellĄ¯s secretory response was measured by the percent of amylase released from the cells in the incubation medium Calcium receptor antagonists, inositol trisphosphate (IP3) receptor blockers, mitogen activated protein kinase inhibitors and specific nicotinic receptor antagonists were used to confirm the involvement of calcium in this process. Results Nicotine exposure induced enhanced secretory response in primary cells. These responses remained unaffected by mitogen activated protein kinases (MAPKĄ¯s) inhibitors. The effects, however, have been completely abolished by nicotinic receptor antagonist, calcium channel receptor antagonists and inositol trisphosphate (IP3) receptor blockers. Conclusions The data suggest that calcium activated events regulating the exocytotic secretion are affected by nicotine as shown by enhanced functional response which is inhibited by specific antagonistsĄ­ The results implicate the role of nicotine in the mobilization of both intra- and extracellular calcium in the regulation of stimulus-secretory response of enzyme secretion in this cell system. We conclude that nicotine plays an important role in promoting enhanced calcium levels inside the acinar cell.
%K Isolated pancreatic acinar cell
%K Nicotine
%K Receptor blocker
%K Calcium channel antagonists
%K MAPK kinase inhibitors
%U http://www.tobaccoinduceddiseases.com/content/11/1/1