%0 Journal Article
%T Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems
%A Antov Mirjana G.
%A Jugovi£¿ Branimir Z.
%A Gvozdenovi£¿ Milica M.
%A Kne£¿evi£¿-Jugovi£¿ Zorica D.
%J Acta Periodica Technologica
%D 2012
%I Faculty of Technology, Novi Sad
%R 10.2298/apt1243151a
%X This study is concerned with the partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems. In the system of 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500,000/80% (w/w) crude enzyme at the pH 5, 100%, yield of cellulolytic activity from Penicillium sp. in the top phase was achieved in a single extraction step. Addition of KH2PO4 to this system at a concentration of 15 mmol/L improved the purification factor in the top phase for cellulolytic activity from crude preparation to a value of 2.6, although it had an adverse effect on the yield in the same phase. [Projekat Ministarstva nauke Republike Srbije, br. 46010]
%K aqueous two-phase system
%K cellulolytic activity
%K partitioning
%K purification
%U http://www.doiserbia.nb.rs/img/doi/1450-7188/2012/1450-71881243151A.pdf