%0 Journal Article
%T Pr谷-condicionamento das sementes de mamoneira para o teste de tetraz車lio = Preconditioning of castor bean seeds for the tetrazolium test
%A Carolina Maria Gaspar-Oliveira
%A Cibele Chalita Martins
%A Joˋo Nakagawa
%J Acta Scientiarum : Agronomy
%D 2011
%I 
%X O objetivo do trabalho foi padronizar a metodologia de pr谷-condicionamento das sementes de mamoneira (Ricinus communis L.) para a avalia  o do potencial fisiol車gico pelo teste de tetraz車lio. Testaram-se os seguintes m谷todos de pr谷-condicionamento: sementes com tegumento entre papel umedecido a 30, 35 e 40oC por 6, 8, 10, 12, 14, 16 e 18h; sementes sem tegumento entre papel umedecido e sementes com tegumento imersas em 芍gua a 25, 30, 35 e 40o C por 1, 2, 3, 4, 5 e 6h. Ap車s o pr谷-condicionamento, removeu-se o tegumento das sementes, que foram cortadas no sentido do comprimento e imersas na solu  o de tetraz車lio a 0,2%, por 120 min., a 35oC. Avaliaram-se a porcentagem de germina  o das sementes, o teor de 芍gua, antes e ap車s a embebi  o, e a uniformidade na colora  o das sementes ap車s o teste de tetraz車lio. O delineamento experimental foi inteiramente casualizado, e a compara  o de m谷dias realizada pelo teste de Tukey a 5% de probabilidade. Concluiu-se que o pr谷-condicionamento para o teste detetraz車lio deve ser realizado nas sementes de mamoneira com tegumento, entre papel toalha umedecido, a 35oC por 12h para que os resultados desse teste assemelhem-se aos obtidos no teste de germina  o. This research had the objective of standardizing the methodology for preconditioning of castor bean (Ricinus communis L.) seeds for the evaluation of their physiological potential by the tetrazolium test. The evaluated seed preconditioning methods were: seeds with coatbetween moist paper towel at 30, 35 and 40oC for 6, 8, 10, 12, 14, 16 and 18 hours; and seeds without coat between moist paper towel; and seeds with coat immersed in water, at 25, 30, 35 and 40oC for 1, 2, 3, 4, 5 and 6 hours. After preconditioning, the seed coat was removed, the seeds were cut lengthwise, and immersed in tetrazolium solution at a concentration of 0.2% for 120 minutes at 35oC. The seeds＊ germination percentage, moisture content before and after preconditioning, and staining uniformity after the tetrazolium were evaluated. The statistical design was completely randomized, and themeans comparisons were accomplished by the Tukey test at 0.05 level of probability. It was concluded that castor bean seeds should be preconditioned with coat between moist paper towel at 35oC for 12 hours, so that the results of the tetrazolium test will be similarto those obtained in the germination test.
%K viabilidade
%K mamona
%K metodologia
%K potencial fisiol車gico
%K viability
%K castor bean
%K methodology
%K physiological potential
%U http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6025/6025