%0 Journal Article
%T Avalia  o da resist那ncia a antibi車ticos de bact谷rias isoladas de efluente hospitalar = Evaluation of resistance to antibiotics of bacteria isolated from hospital effluents
%A Elenice Tavares Abreu
%A Joˋo Adelmo Pretto
%A ˋngelo de Oliveira Caleare
%A C谷lia Regina Granhen Tavares
%J Acta Scientiarum : Technology
%D 2010
%I Editora da Universidade Estadual de Maring芍
%X O uso intensivo de antibi車ticos est芍 entre as principais causas de resist那ncia bacteriana. O objetivo deste estudo foi investigar se o esgoto do Hospital Universit芍rio Regional de Maring芍 (HUM) apresentava bact谷rias patog那nicas resistentes a antibi車ticos. Asamostras foram coletadas em dois pontos, um de todo o hospital e outro do Hemocentro. Foram semeados 100 米L nas dilui  es 10-2, 10-3 e 10-4 nos meios de culturas 芍gar Mac Conkey, 芍gar Salmonella-Shigella, 芍gar Manitol Salgado e 芍gar Sabouraud Dextrose. As amostras foram submetidas 角 identifica  o bacteriana e ao antibiograma. A identifica  o bacteriana se baseou na bacterioscopia por colora  o de Gram e provas bioqu赤micas. Os antibiogramas foram feitos de acordo com a metodologia de difus o de disco em 芍gar M邦eller-Hinton, e foram utilizados 12 antibi車ticos nesses testes. Foram isoladas eidentificadas 39 amostras, e dentre estas, 18 esp谷cies apresentaram resist那ncia e moderada resist那ncia pelo menos para um dos antibi車ticos. Bact谷rias isoladas e identificadas como K. pneumoniae, E.coli, K. pneumoniae, E. cloacae e C. freundii apresentaram resist那ncia para quase todos os antibi車ticos testados. Verificou-se que o HUM lan a, na rede p迆blica coletora de esgoto, bact谷rias com multirresist那ncia a determinados antibi車ticos. Portanto, torna-se necess芍ria a implanta  o de sistema eficiente de tratamento de efluentes no HUM. The massive use of antibiotics is among the leading causes of bacterial resistance. The objective of this study is to investigate whether the sewage of the Maring芍 Regional University Hospital (HUM) has pathogenic bacteria resistant to antibiotics. The samples were collected at two sites, one from the entire hospital and the other from the blood bank. 100 mL were inserted in dilutions of 10-2, 10-3 and 10-4 in cultures Mac Conkey Agar, Salmonella-Shigella Agar, Mannitol Salt Agar and Sabouraud Dextrose Agar. The samples were subjected to bacterial identification and antibiogram. The identification was based on bacterioscopy by Gram staining, and biochemical evidence. The antibiograms were made in accordance with the disk diffusion methodology in M邦eller-Hinton agar, and 12 antibiotics were used in these tests. They were isolated and 39 samples were identified; of these, 18 species showed resistance and moderate resistance to at least one antibiotic. Bacteria isolated and identified as K. pneumoniae, E. coli, K. pneumoniae, E. cloacae andC. freundii showed resistance to almost all tested antibiotics. It was found that the HUM releases bacteria with multi-resistant to certain antibiotics int
%K resist那ncia bacteriana
%K efluente hospitalar
%K antibi車ticos
%K bacterial resistance
%K hospital effluent
%K antibiotics
%U http://periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7453/7453