%0 Journal Article
%T Genome-wide analysis of Acetivibrio cellulolyticus provides a blueprint of an elaborate cellulosome system
%A Bareket Dassa
%A Ilya Borovok
%A Raphael Lamed
%A Bernard Henrissat
%A Pedro Coutinho
%A Christopher L Hemme
%A Yue Huang
%A Jizhong Zhou
%A Edward A Bayer
%J BMC Genomics
%D 2012
%I BioMed Central
%R 10.1186/1471-2164-13-210
%X Comprehensive analysis of the A. cellulolyticus draft genome sequence revealed a very sophisticated cellulosome system. Compared to C. thermocellum, the cellulosomal architecture of A. cellulolyticus is much more extensive, whereby the genome encodes for twice the number of cohesin- and dockerin-containing proteins. The A. cellulolyticus genome has thus evolved an inflated number of 143 dockerin-containing genes, coding for multimodular proteins with distinctive catalytic and carbohydrate-binding modules that play critical roles in biomass degradation. Additionally, 41 putative cohesin modules distributed in 16 different scaffoldin proteins were identified in the genome, representing a broader diversity and modularity than those of Clostridium thermocellum. Although many of the A. cellulolyticus scaffoldins appear in unconventional modular combinations, elements of the basic structural scaffoldins are maintained in both species. In addition, both species exhibit similarly elaborate cell-anchoring and cellulosome-related gene- regulatory elements.This work portrays a particularly intricate, cell-surface cellulosome system in A. cellulolyticus and provides a blueprint for examining the specific roles of the various cellulosomal components in the degradation of complex carbohydrate substrates of the plant cell wall by the bacterium.
%K Cellulosomics
%K Clostridium thermocellum
%K Scaffoldin
%K Cohesin
%K Dockerin
%U http://www.biomedcentral.com/1471-2164/13/210/abstract