%0 Journal Article
%T Enhanced Production and Extracellular Activity of Commercially Important Amylolytic Enzyme by a Newly Isolated Strain of Bacillus. sp. AS-1
%A Shah Ali UI Qader
%A Saeeda Bano
%A Samina Iqbal
%A Noman Syed
%J T¨¹rk Biyokimya Dergisi
%D 2006
%I Turkish Biochemical Society
%X Studies on the optimum conditions for the production of ¦Á-amylase were carried outwith a newly isolated bacterial strain of bacillus sp.AS-1. The optimum temperaturefor amylase production was detected as 35¡ãC. ¦Á -Amylase production occurred atpH 5.0-9.0 with a maximum at pH 7.0. The optimal pH and temperature values forextracellular activity were 7.5 and 50¡ãC respectively. Effect of different salts werenoted and it was found that CaCl2 with concentration of 0.2g/l played an importantrole for optimum production and stability of alpha amylase in the fermentationmedium. Starch with a concentration of 20 g/l was a good source for the enzymesynthesis. The levels of the ¦Á-amylase production detected in culture supernatantsvaried greatly with the type of carbon source used. Lactose, soluble starch andglucose stimulated ¦Á-amylase production. Effect of different nitrogen sourcesrevealed that peptone increase the enzyme yield. The concentration of yeast extractwas an important factor for the ¦Á-amylase synthesis by the isolate. The activity ofthe enzyme increased between 2 and 4 g/l yeast extract concentrations with anoptimum of 4 g/l. The optimal concentration of peptone for the production of ¦Á-amylase was detected as 10g/l.
%K ¦Á-Amylase
%K Bacillus sp.
%K Starch
%K CaCl2
%K Nitrogen source
%U http://www.turkjbiochem.com/2006/135.pdf