%0 Journal Article
%T Targeted Fluoromagnetic Nanoparticles for Imaging of Breast Cancer MCF-7 Cells
%A Mostafa Heidari Majd
%A Jaleh Barar
%A Davoud Asgari
%A Hadi Valizadeh
%J Advanced Pharmaceutical Bulletin
%D 2013
%I Tabriz University of Medical Sciences
%R 10.5681/apb.2013.031
%X Purpose: To achieve simultaneous imaging and therapy potentials, targeted fluoromagnetic nanoparticles were synthesized and examined in human breast cancer MCF-7 cells. Methods: Fe3O4 nanoparticles (NPs) were synthesized through thermal decomposition of Fe(acac)3. Then, magnetic nanoparticles (MNPs) modified by dopamine-poly ethylene glycol (PEG)-NH2; finally, half equivalent fluorescein isothiocyanate (FITC) and half equivalent folic acid were conjugated to one equivalent of it. The presence of Fe3O4-DPA-PEG-FA/FITC in the folate receptor (FR) positive MCF-7 cells was determined via fluorescent microscopy to monitor the cellular interaction of MNPs. Results: FT-IR spectra of final compound confirmed existence of fluorescein on folic acid grafted MNPs. The Fe3O4-DPA-PEG-FA/FITC NPs, which displayed a size rang about 30-35 nm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), were able to actively recognize the FR-positive MCF-7 cells, but not the FR-negative A549 cells. Conclusion: The uniform nano-sized Fe3O4-DPA-PEG-FA/FITC NPs displayed great potential as theranostics and can be used for targeted imaging of various tumors that overexpress FR.
%K Magnetic nanoparticles
%K Folate receptor
%K Breast cancer
%K MCF-7 cells
%K Internalization
%U http://journals.tbzmed.ac.ir/PDF/APB/Manuscript/APB-3-189.pdf