%0 Journal Article
%T AN INCREASE OF THE TOTAL SOLIDIFICATION TEMPERATURE IN SALT MEDIA BY DEXTRAN PROTECTION
%A Todor Donev and Snezhana Dimitrova
%J Journal of Culture Collections
%D 1998
%I 
%X An investigation was performed on the possibilities for an alteration of the total solidification temperature when preserving concentrated salt media (MBM, MEM, 199, HAM), used for the maintenance of animal viruses and cell cultures. It had been established that by the use of dextran 40000, this temperature could be increased with about 20ˇăC. A protection in 3.6% concentration was recommended and the freezing to minus 40ˇăC might be performed directly in the refrigerator for storage. The temperature should not be raised over minus 32ˇăC during the stockage.
%U http://www.bioline.org.br/request?cc98012