%0 Journal Article
%T Influence of variable domain glycosylation on anti-neutrophil cytoplasmic autoantibodies and anti-glomerular basement membrane autoantibodies
%A Peng-Cheng Xu
%A Shen-Ju Gou
%A Xiao-Wei Yang
%A Zhao Cui
%A Xiao-Yu Jia
%A Min Chen
%A Ming-Hui Zhao
%J BMC Immunology
%D 2012
%I BioMed Central
%R 10.1186/1471-2172-13-10
%X Twenty-seven patients with serum positive anti-neutrophil cytoplasmic autoantibodies (ANCA) against myeploperoxidase (MPO) or proteinase 3 (PR3), or autoantibodies against glomerular basement membrane (GBM) were included. Total IgG was isolated and separated into non-SNA-binding and SNA-binding fractions with SNA affinity chromatography. Antigen-specific IgG was purified by immunoaffinity chromatography.At the same concentration of IgG, the antigen binding level of non-SNA-binding IgG was significantly lower than that of SNA-binding IgG for MPO-ANCA (absorbance value at 405 nm, 0.572 ¡À 0.590 vs. 0.962 ¡À 0.670, P < 0.001) and for PR3-ANCA (0.362 ¡À 0.530 vs. 0.560 ¡À 0.531, P = 0.003). The antigen binding level of non-SNA-binding IgG was significantly higher than that of SNA-binding IgG for anti-GBM antibodies (1.301 ¡À 0.594 vs. 1.172 ¡À 0.583, P = 0.044). The level of variable region glycosylation of total IgG was significantly lower than that of affinity-purified MPO-ANCA (1.021 ¡À 0.201 vs. 1.434 ¡À 0.134, P = 0.004). The level of variable region glycosylation of total IgG was significantly higher than that of affinity-purified anti-GBM antibodies (1.034 ¡À 0.340 vs. 0.734 ¡À 0.333, P = 0.007). The SNA-binding fraction of MPO-ANCA-containing IgG and PR3-ANCA-containing IgG induced higher levels of neutrophil oxygen radical production than the corresponding non-SNA-binding fractions (P < 0.001 and P = 0.043, respectively). The level of variable region glycosylation of affinity-purified MPO-ANCA was higher in active AAV than the same patients in remission (P = 0.001).Characteristics of variable region glycosylation of ANCA and anti-GBM antibodies were different from that of total IgG, which might influence the antigen-binding ability of these antibodies. Variable region glycosylation of ANCA might influence the effect of ANCA-induced neutrophils respiratory burst.Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV) comprise granulomatosis with polyangi
%K Glycosylation
%K Variable region
%K ANCA
%K Anti-GBM
%U http://www.biomedcentral.com/1471-2172/13/10