%0 Journal Article
%T Isolation, Molecular Identification and Evaluation of Antifungal Effects of the Bacilli Isolated from Rhizospheric Soil of Gorgan Region against Tricophyton Mentagrophytes
%A Pordeli
%A HR. (PhD)
%A Hashemi Hazaveh
%A SJ. (PhD)
%A Jamshidian
%A M. (PhD)
%A Bayat
%A M. (PhD)
%J Medical Laboratory Journal
%D 2013
%I Golestan University of Medical Sciences
%R http://www.goums.ac.ir/mljgoums/index.php?slc_lang=en&sid=1
%X Background and Objective: Soil bacteria, particularly Bacillus genus have thepotential of producing a range of bioactive substances with antimicrobial andantifungal properties. They have the ability to produce hundreds of active andeffective biologic compound against microorganisms. Therefore, it seems to be aproper candidate in the biocontrol of fungal pathogenesis.Material and Methods: In this study, soil samples were collected from differentparts of Gorgan in order to isolate Bacillus and to determine their antifungalactivity against T.mentagrophytes. The Isolates that had the highest antifungaleffects were analyzed by PCR and 16s rRNA sequencing.Results: Of 54 strains, 14 have antifungal activity. The Isolates, S4 and S12,identified as B.cereus and B.thuringiensis respectively show the highestantidermatofit effect. These isolates based on 16s rRNA sequence analysis show97% homology with Bacillus cereusstrain KU4 and Bacillus thuringiensisstrainucsc27.Conclusion: According to the results, it seems that the soil Bacilli have biocontrolpotential against dermatophytic agents such as T.mentagrophytes.Keywords: Antifungal effects; Bacillus; Rhizospheric soil; T.mentagrophytes
%K Antifungal effects
%K Bacillus
%K Rhizospheric soil
%K T.mentagrophytes
%U http://www.goums.ac.ir/mljgoums/browse.php?a_id=271&slc_lang=en&sid=1&ftxt=1