%0 Journal Article
%T Rapid DNA extraction of bacterial genome of Staphylococcus aureus using laundry detergents and assessment of the efficiency of DNA in downstream process using PCR
%A Mousazade moghadam M
%A Babavalian H
%A Mirnejad R
%A Shakeri F
%J Medical Laboratory Journal
%D 2012
%I Golestan University of Medical Sciences
%R http://www.goums.ac.ir/mljgoums/index.php?&slct_pg_id=10&sid=1&slc_lang=en
%X Background and objectives: Genomic DNA extraction of bacterial cells is ofprocesses performed normally in most biological laboratories; therefore,various methods have been offered, manually and kit, which may be timeconsuming and costly. In this paper, genomic DNA extraction ofStaphylococcus aureus was investigated using some laundry detergent brandsavailable in Iran to achieve a rapid and cost effective method.Material and Methods: five-enzyme Taj brand, three-enzyme Saftlan brand,and Darya and Pak brands without enzyme were used in the concentrations of10, 20, 40, 80 mg/L. Afterwards, in order to evaluate the efficiency ofextracted DNA in downstream processing, PCR test was performed for femAgene in the genome of Staphylococcus aureus.Results: DNA extraction using different concentrations of the brands showthat extracted DNA using 40 mg/L Saftlan and Taj brand powders have thebest results according concentration (¦Ìg/ml) and purity (A260/A280)parameters. These parameters are 387.5; 1.88 (Taj), 254.1; 2.80 (Softlan),396.6; 1.95 (Manual) and 423.3; 2.2 (Kit), respectively. Afterward, the PCRtest results by show that DNA extraction using laundry detergents has noeffect on its efficiency in order to be used in downstream processes.Conclusion: These results indicate that the proper concentrations of laundrydetergents can be used to extract genomic DNA with similar efficiency to kitand manual extraction methods.Key words: Bacterial genome, DNA extraction, laundry powder, PCR,Staphylococcus aureus
%K Bacterial genome
%K DNA extraction
%K laundry powder
%K PCR
%K Staphylococcus aureus
%U http://www.goums.ac.ir/mljgoums/browse.php?a_id=189&slc_lang=en&sid=1&ftxt=1