%0 Journal Article
%T Development and validation of a RP- HPLC method for the quantitation studies of bromadiolone in Ratitox F
%A Elena Gabriela Oltean
%A Nica
%A A.
%J Medicamentul Veterinar
%D 2011
%I Romanian National Association of the Veterinary Products Manufacturers
%X An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of bromadiolone (hydroxycoumarins) in Ratitox F product ¨C rodenticide. HPLC separation was carried out by reversed phase chromatography ODS 2 Hypersil C18 (250 mm x 4.6 mm i.e.; 5 ¨¬m particle size), held in thermostat at 25¡ãC. The mobile phase consisted of methanol/0.1% aqueous solution phosphoric acid (90/10v/v), with a flow rate of 1 ml/min and with UV detection at 265 nm. In order to validate the method, the following parameters have been investigated- linearity (r2 = 0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of Ratitox F ¨C rodenticide.
%K bromadiolone
%K Ratitox F
%K reversed phase high performance liquid chromatography RP-HPLC UV
%U http://www.veterinarypharmacon.com/docs/999-2011-ART.%206.%20eng.pdf