%0 Journal Article
%T Silencing the polarity protein Scribble in murine hematopoietic progenitor cells
%A Ann Marie Macara
%A Kelly Pike
%A Anthony J Pawson
%J University of Toronto Journal of Undergraduate Life Sciences
%D 2009
%I University of Toronto
%X It has been shown that the polarity protein Scribble is required for T-cell migration, which itself is important for lymphocyte development. To study its effects on B-cell development and migration, Scribble gene expression was silenced using a self-inactivating retroviral expression vector that produces a small hairpin RNA in murine hematopoietic progenitor cells (HPCs). Infected HPCs were then allowed to differentiate into B lymphocytes on an OP9 bone marrow stromal cell line. Silencing of Scribble expression in HPCs did not inhibit commitment to the B-cell lineage. However, these B lineage cells failed to survive in culture over a period of three weeks. The loss of B lymphocytes may indicate a role for the protein Scribble in B-cell proliferation, survival, and cell death.
%K B-cell development
%K hairpin RNA
%K hematopoietic progenitor cell
%K retroviral expression vector
%K Scribble
%U http://juls.library.utoronto.ca/index.php/juls/article/view/11153