%0 Journal Article
%T MicroRNA profiling of a CD133+ spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line
%A Eun Ji Nam
%A Maria Lee
%A Ga Won Yim
%A Jae Hoon Kim
%A Sunghoon Kim
%A Sang Wun Kim
%A Young Tae Kim
%J BMC Medical Genomics
%D 2012
%I BioMed Central
%R 10.1186/1755-8794-5-18
%X Initially, we confirmed the enrichment of the OVCAR3 CD133 subpopulation by evaluating in vitro anchorage-independent growth. After obtaining a subpopulation of CD133+ OVCAR3 cells with£¿>£¿98% purity via cell sorting, miRNA microarray and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to evaluate its miRNA profile.We found 37 differentially expressed miRNAs in the CD133+ spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b, and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181.Our results indicate that dysregulation of miRNA may play a role in the stem cell-like properties of ovarian CSCs.
%K MicroRNA
%K Cancer stem cell
%K Ovarian cancer
%K CD133
%K OVCAR3
%K Chemoresistance
%U http://www.biomedcentral.com/1755-8794/5/18/abstract