%0 Journal Article
%T Callus formation in vitro and internodal stem apices in savory = Calog那nese in vitro de segmentos apicais caulinares e internodais em segurelha (Satureja hortensis L.)
%A Marcio Carlos Navroski
%A Daniel Arthur Gaklik Waldow
%A Mariane de Oliveira Pereira
%A Alessandra de Oliveira Pereira5
%J Agro@mbiente On-line
%D 2012
%I Universidade Federal de Roraima
%X We sought to evaluate with this work different growth regulators on callus formation in shoot apical and internodal stem segments of Satureja hortensis. The explants were isolated from in vitro seedlings and cultured on MS nutrient medium supplemented with NAA (0 and 1 米M) and BAP (0, 5, 10, 15 and 20 米M). The presence of auxin NAA gave higher marks to the calluses at 30 days of evaluation, these notes also increased with the addition of BAP. There were significant interactions between factors in evaluating NAA and BAP for 60 days, both in apical stem segments as in internodal stem segments. To stemapices percentage of friable callus tends to decrease with increasing concentration of BAP. As for compact calluses increased BAP leads to an increase in the percentage of this type of callus. The presence of NAA increased callus formation in both friable and compact calluses on. This trend was also observed in internodal stem segments. The callus formation was highly rhizogenic observed in the presence of NAA, hardly occurs in the absence of auxin. The use of BAP is recommended in case of regeneration of plants through micropropagation, if the goal is the production of metabolites, the use of BAP can be harmful by reducing the production of friable callus.= Buscou-se com este trabalho avaliar diferentes reguladores de crescimento sobre a calog那nese em segmentos caulinares apicais e internodais de Satureja hortensis L.. Os explantes foram isolados de plantulas germinadas in vitro e cultivados em meio nutritivo MS acrescido de ANA (0 e 1 米M) e de BAP (0; 5; 10; 15 e 20 米M). A presen a da auxina ANA proporcionou notas mais altas para os calos aos 30 dias de avalia  o, estas notas tamb谷m aumentaram com o acr谷scimo da citocinina BAP. Houve intera  es significativas entre os fatores ANA e BAP na avalia  o aos 60 dias, nos dois tipos de segmentos caulinares(apicais e internodais). Nos segmentos apicais caulinares a porcentagem de calos fri芍veis tendeu a diminuir com o aumento da concentra  o de BAP. J芍 nos calos compactos o aumento de BAP ocasionou um aumento da porcentagem deste tipo de calo. A presen a de ANA elevou a forma  o tanto em calos fri芍veis como em calos compactos. Esta tend那ncia tamb谷m foi observada em segmentos caulinares internodais. A forma  o de calos rizog那nicos foi altamente observada na presen a de ANA, ase n o ocorrendo na aus那ncia desta auxina. A utiliza  o de BAP 谷 recomendada em caso de regenera  o de plantas por micropropaga  o, no entanto, se o objetivo for 角 produ  o de metab車litos, a utiliza  o de BAP pode ser prejudicial por diminuir a
%K Tissue culture
%K Secondary metabolites
%K Growth regulators. = Cultura de tecidos
%K Metab車litos secund芍rios
%K Reguladores de crescimento.
%U http://revista.ufrr.br/index.php/agroambiente/article/view/698