%0 Journal Article
%T Presencia de Phomopsis viticola (Saccardo) Saccardo, agente causal de excoriosis, en vi edos de C車rdoba, Argentina Presence of Phomopsis viticola (Saccardo) Saccardo, causal agent of excoriosis, in vineyards of C車rdoba, Argentina
%A C.I. Cragnolini
%A G.J. March
%A A.I. Viglianco
%A R.J. Novo
%J Agriscientia
%D 2009
%I 
%X Desde 2001 se observan en vi edos de C車rdoba, Argentina, hojas de Vitis vinifera y V. labrusca con manchas clor車ticas y centro necr車tico. Las plantas presentan nervaduras, pec赤olos, raquis y entrenudos con puntos necr車ticos que al confluir agrietan el tejido cortical. Las bayas pueden marchitarse y desprenderse. En invierno los sarmientos toman color blanquecino con manchas negras, y en ellos y en 車rganos secos aparecen picnidios con cirros blanco-crema con conidios alfa y beta. El objetivo fue confirmar la presencia de Phomopsis viticola (excoriosis) y cuantificar su incidencia. Se realizaron aislamientos a partir de pec赤olos, brotes, zarcillos, bayas y sarmientos que produjeron micelio claro con picnidios y conidios alfa (7,5m㊣1,5 x 2,8m㊣2) y beta (19m㊣1 x 1,2m㊣0,5) o s車lo alfa, t赤picos de P. viticola. La identificaci車n fue confirmada mediante secuenciaci車n molecular. En brotes de las variedades Malbec y Pinot noir inoculados con 3 10(4) conidios/mL, las hojas mostraron lesiones y picnidios 8 d赤as despu谷s, y las bayas cirros amarillos a los 15-18 d赤as. P. viticola (Sacc.) Sacc. fue reaislado de los cirros y las colonias produjeron picnidios y conidios caracter赤sticos 10-20 d赤as despu谷s. Se detect車 excoriosis en 80% de los vi edos evaluados, con mayor incidencia en vi edos a osos de las variedades Pinot noir e Isabella. Leaves with chlorotic spots and necrotic centers have been observed in Vitis vinifera and V. labrusca in vineyards of C車rdoba, Argentina, since 2001. Plants exhibit veins, petioles, rachises, and internodes with necrotic spots that coalesce to form cracks in the cortical tissue. Grape berries can shrivel and fall off. Winter canes became whitish with black spots; pycnidia appear in canes and in dry organs, exuding whitish-cream cirri with alpha and beta conidia. The aim of this work was to confirm the presence of Phomopsis viticola (excoriosis) and quantify its incidence. Isolates obtained from petioles, shoots, tendrils, berries and canes produced light-coloured mycelium with pycnidia and alpha (7.5m㊣1.5 x 2.8m㊣2) and beta (19m㊣1 x 1.2m㊣0.5) conidia or only alpha conidia, typical of P. viticola. Identification was confirmed by molecular sequencing. In shoots of the varieties Malbec and Pinot noir inoculated with 3 10(4) conidia/mL, leaves exhibited lesions and pycnidia after eight days, and berries showed yellow cirri 15-18 days after inoculation. P. viticola (Sacc.) Sacc. was reisolated from the cirri and the colonies produced typical pycnidia and conidia 10-20 days later. Excoriosis was present in 80% of grapevines evaluated with hig
%K Excoriosis
%K Necrosis de la vid
%K Tiz車n del tallo y mancha de la hoja
%K Phomopsis viticola
%K Vitis vinifera
%K Vitis labrusca
%K Excoriosis
%K Necrosis of grapevine
%K Phomopsis cane blight and leaf spot
%K Phomopsis viticola
%K Vitis vinifera
%K Vitis labrusca
%U http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2009000200001