%0 Journal Article
%T Production of haploid plants from ten hybrids of bread wheat (Triticum aestivum L.) through wide hybridization with maize (Zea mays L.) Producci車n de plantas haploides a partir de 10 h赤bridos de trigo para pan (Triticum aestivum L.) mediante hibridaci車n interespec赤fica con ma赤z (Zea mays L.)
%A L.E. Torres
%A P. Bima
%A R. Maich
%A O.J.J. Badiali
%J Agriscientia
%D 2010
%I 
%X The aim of this work was to obtain haploid plants of bread wheat through wide hybridization with maize. The experimental material included ten bread wheat hybrids (female parent) and one population of maize (pollen donor). Two assays were carried out in two different seasons (summer and winter). Wheat spikes were manually emasculated, each spike was pollinated twice with fresh pollen of maize and a solution of 2,4-D (100 mg l-1) was sprayed on pollinated florets and injected in the upper internode. Fifteen and 21 days after pollination caryopses were removed and surface sterilized. Embryos were cultured in tubes containing B5 medium. The ten hybrid combinations produced caryopses, but only eight of these hybrids produced embryos and, in six of them, the recovered embryos developed into haploid plantlets. The results showed that there is genotypic influence of the wheat parents on the percentage of haploid embryo formation, in accordance with the results obtained by other authors. Regardless of the genotype, the sowing season and the harvest date, 69.4% of the pollinated flowers gave place to the formation of caryopses, 5.5% of these caryopses developed into presumably haploid embryos (for their morphological phenotypes) and 26.1 % of the recovered embryos developed into haploid plantlets. El objetivo del presente trabajo fue obtener plantas haploides de trigo para pan mediante hibridaci車n interespec赤fica con ma赤z. Se utilizaron 10 h赤bridos de trigo para pan (madre) y una poblaci車n de ma赤z (donante de polen); se llevaron a cabo dos ensayos en distintas estaciones de cultivo. Cada espiga de trigo fue emasculada manualmente y polinizada dos veces con polen fresco de ma赤z; las flores polinizadas se pulverizaron con una soluci車n de 2,4-D (100 mg l-1), la que tambi谷n se inyect車 en la base de la espiga. Las semillas se cosecharon a los 15 y 21 d赤as posteriores a la polinizaci車n. Los embriones recuperados se colocaron en tubos conteniendo medio de cultivo B5. Las 10 combinaciones h赤bridas produjeron semillas, de ocho de los h赤bridos se recuperaron embriones y en seis de ellos los embriones desarrollaron plantas haploides. Estos resultados muestran que existe influencia del genotipo del trigo sobre el porcentaje de formaci車n de embriones haploides. Independientemente del genotipo materno, la estaci車n de cultivo y la edad de los embriones recuperados, 69,4% de las flores polinizadas formaron caryopses, 5,5% de los caryopses formados desarrollaron embriones presuntamente haploides (por sus fenotipos morfol車gicos) y 26,1% de los embriones recuperados desarrollaron
%K Trigo para pan
%K Plantas haploides
%K Hibridaci車n interespec赤fica
%K Polen de ma赤z
%K Bread wheat
%K Haploid plants
%K Wide hybridization
%K Maize pollen
%U http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2010000200003