%0 Journal Article
%T Differences in wing melanization and pigmentation pattern in Drosophila buzzatii (Diptera: Drosophilidae) under chemical stress Diferencias en la melanizaci車n y el patr車n de pigmentaci車n alar en Drosophila buzzatii (Diptera: Drosophilidae) bajo estr谷s qu赤mico
%A Nicol芍s Mongiardino Koch
%A Esteban Hasson
%A Ignacio M. Soto
%J Revista de la Sociedad Entomolˋ3gica Argentina
%D 2012
%I Sociedad Entomol車gica Argentina
%X Recently, the genetic basis and developmental mechanisms underlying the process of melanization have become progressively elucidated, allowing us to study the evolutionary processes that govern the huge variability of pigmentation observed in nature. However, environmental factors contributing to such variability have received little attention, even though they might have profound ecological consequences. Here we describe a method for analyzing the wing melanization patterns of drosophilids in both qualitative and quantitative ways. We test this method on wings of Drosophila buzzatii Patterson & Wheeler individuals, reared in control and alkaloid-enriched mediums. The alkaloids were extracted from the secondary host of these flies, Echinopsis terscheckii (Parm. ex Pfeiff.) Friedrich & Rowley, and their effect on wing pigmentation is analyzed, bearing in mind the adverse effects that these compounds have on the development of D. buzzatii. Alkaloid-reared flies were shown to attain a higher degree of wing melanization, accompanied with differences in the spatial distribution of the pigment. Modifications at both metabolic and gene regulatory levels are proposed to explain the changes that chemical stressful conditions are shown to induce in this character. We propose the utilization of this procedure in studies of environmental dependence of pigmentation. Recientemente, hemos avanzado en nuestra comprensi車n de las bases gen谷ticas y los mecanismos subyacentes al proceso de melanizaci車n durante el desarrollo, lo que permite el estudio de los procesos evolutivos que gobiernan la variabilidad de pigmentaci車n presente en la naturaleza. Sin embargo, los factores ambientales que contribuyen a dicha variabilidad han recibido poca atenci車n, a迆n cuando pueden conllevar profundas consecuencias a nivel ecol車gico. En este trabajo, presentamos un m谷todo para analizar los patrones de melanizaci車n alar de drosof赤lidos, de forma cualitativa y cuantitativa. Ponemos a prueba esta metodolog赤a comparando las alas de Drosophila buzzatii Patterson & Wheeler, criadas en medio control y con el agregado de alcaloides. Los alcaloides fueron extra赤dos del hu谷sped secundario de estas moscas, Echinopsis terscheckii (Parm. ex Pfeiff.) Friedrich & Rowley, los cuales, se sabe, presentan efectos negativos en el desarrollo de D. buzzatii. Las moscas criadas en un medio enriquecido con alcaloides mostraron una mayor melanizaci車n alar, junto con diferencias en la distribuci車n espacial del pigmento. Planteamos modificaciones a nivel metab車lico y de expresi車n g谷nica para explicar los cambios q
%K Drosophila buzzatii
%K Melanina
%K Ecotoxicolog赤a
%K Alcaloides
%K Stress
%K Drosophila buzzatii
%K Melanin
%K Ecotoxicology
%K Alkaloids
%K Stress
%U http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802012000100008