%0 Journal Article
%T Immunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins
%A Selvarayar Arunkumar
%J Veterinary Research Forum
%D 2012
%I Urmia University
%X Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37  C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a single reactive band at 66 kDa. In immunization trial, sheep were administered intramuscularly with 500 ¦Ìg of thiol-purified excretory/secretory antigen along with montanide as adjuvant on day 0, 30 and 60. On ELISA, it was observed that the mean absorbance values were significantly (p ¡Ü 0.01) higher up to 20 weeks post immunization in Group-I (purified antigen) compared to Group- II (unimmunized control). Further, the mean EPG values was lower in Group I (200.00 ¡À 40.82 to 400.00 ¡À 91.29) than Group II (2200.00 ¡À 108.01 to 5100.00 ¡À 169.56) and the percentage reduction in mean fecal egg counts was 88.50%. Similarly, the mean abomasal worm counts was lower in Group I (808.33 ¡À 78.29) than Group II (3280.00 ¡À 147.19) and the percentage reduction in mean abomasal worm count was 75.40%.
%K Haemonchus contortus
%K Excretory/secretory antigen
%K Antibody
%K Fecal egg count
%K Sheep
%U www.urmia.ac.ir/vrf/Shared%20Documents/pdf/vol-3%20no-4/239-244-0145.pdf