%0 Journal Article
%T Dengue viruses exhibit strain-specific infectivity and entry requirements in vitro
%A Barr KL
%A Anderson BD
%J Virus Adaptation and Treatment
%D 2013
%I Dove Medical Press
%X Kelli L Barr, Benjamin D Anderson Department of Environmental and Global Health, College of Public Health and Health Professions and the Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA Background: Globally, dengue viruses (DENV) are the most common human arboviral infection and the most important public health threat from mosquito-borne viral pathogens. Recent work has indicated that DENV affinity for cell lines is serotype-specific, which raises challenging questions regarding DENV-host interactions as well as the development of therapeutics and control programs. Methods: We evaluated the infectious capacity of 11 strains of DENV for serotypes 1¨C4 in 17 distinct cell lines. The cell lines were evaluated for virus susceptibility via immunohistochemistry and quantitative reverse transcription polymerase chain reaction. Results: Both methods demonstrated the ability of DENV to replicate in all cell lines, with viral titers ranging from 1 ¡Á 102 to 1 ¡Á 107 infectious units per mL. Cell line susceptibility to DENV infection was strain-specific, with DENV-4 strains infecting more cell lines than the other serotypes. DENV-2 New Guinea C and DENV-4 H241 were detected in more cell lines than any other strains. Viral fusion assays indicated that DENV requirements for fusion are strain-specific. Conclusion: These data indicate that several cell lines can be used to culture and study DENV. The strain-specific susceptibility for certain cell lines may provide a tool for characterizing specific DENV strains and an in vitro platform for the development and optimization of therapeutics, the study of host-pathogen interactions, and ecological studies on the cocirculation of DENV strains in a specific region or individual. Keywords: dengue virus, cell culture, host, fusion
%U http://www.dovepress.com/dengue-viruses-exhibit-strain-specific-infectivity-and-entry-requireme-a12730