%0 Journal Article
%T Renal neutrophil gelatinase associated lipocalin expression in lipopolysaccharide-induced acute kidney injury in the rat
%A Mei Han
%A Ying Li
%A Maodong Liu
%A Yingmin Li
%A Bin Cong
%J BMC Nephrology
%D 2012
%I BioMed Central
%R 10.1186/1471-2369-13-25
%X To induce acute renal injury, rats were treated with lipopolysaccharide (LPS, 3.5 mg/kg, ip), and the location of NGAL mRNA was evaluated by in situ hybridization. Quantitative RT-PCR was also used to determine the dynamic changes in NGAL, tumor necrosis factor ¦Á (TNF¦Á) and interleukin (IL)-6 mRNA expression 1, 3, 6, 12, and 24 hours following LPS treatment. The correlation among NGAL, TNF¦Á and IL-6 was analyzed. Urinary and plasma NGAL (u/pNGAL) levels were measured, and the relationship between humoral NGAL and NGAL expression in the kidney was investigated.Renal function was affected 3¨C12 hours after LPS. NGAL mRNA was significantly upregulated in tubular epithelia at the same time (P < 0.001). The course of NGAL mRNA upregulation occurred in parallel with renal damage. There was a transient increase in TNF¦Á and IL-6 mRNA levels within 3 hours following LPS administration, and a strong correlation between TNF¦Á and NGAL mRNA (r = 0.995, P <0.001) but not with IL-6 mRNA. Both pNGAL and uNGAL levels were markedly increased compared with those in the control group (P < 0.001); however, only uNGAL levels were correlated with NGAL mRNA (r = 0.850, P <0.001).NGAL upregulation is sensitive to LPS-induced renal TNF¦Á increase and injury, which are observed in the tubular epithelia. Urinary NGAL levels accurately reflect changes in NGAL in the kidney.Acute kidney injury (AKI; or acute renal failure) is an important issue for patients during critical care, with sepsis being the most common trigger for AKI in the intensive care unit (ICU) [1-3]. Because of the lack of sensitive and specific biomarkers for indicating renal cell injury, the mortality rates for septic AKI have remained high [1,4]. Recently, genomic, transcriptomic, and proteomic techniques have identified neutrophil gelatinase associated lipocalin (NGAL) as an early marker of AKI [5,6]. NGAL has been investigated in a range of different clinical settings, such as contrast-induced nephropathy, AKI after cardiac s
%K Neutrophil gelatinase associated lipocalin
%K lipopolysaccharide
%K acute kidney injury
%K tumor necrosis factor ¦Á
%U http://www.biomedcentral.com/1471-2369/13/25