%0 Journal Article
%T STUDY OF MIR-200C AND MIR-9 METHYLATION ON PATIENTS WITH BREAST CANCER
%A Anca Florescu
%A Dumitru Cojocaru
%A V. M. FAZIO
%A Paola Parrella
%J Analele £¿tiin£¿ifice Ale Universit£¿£¿ii Alexandru Ioan Cuza din Ia£¿i,Sectiunea II A : Genetica si Biologie Moleculara
%D 2012
%I Alexandru Ioan Cuza University of Iasi
%X Breast cancer is one of the most common causes of death among women worldwide. DNA methylation is essential for normal development and it is associated with a number of key processes including genomic imprinting, X- chromosome inactivation, carcinogenesis. We determined promoter methylation status of miR-9 and miR-200c on DNA obtained from 30 breast cancer cases (mean age of 57¡À14). DNA was extracted from fresh frozen specimens, after ensuring that tumour samples contained more than 70% cancer cell, treated with sodium bisulphite and amplified by using Methylation Specific PCR (MSP). As control four normal breast tissue from healthy women were also analyzed. Methylation at the miR-9 promoter region was detected in 20 out of 31 cases (64%) and none of the normal breast tissues (P=0.026, X-square. Whereas, miR-200c promoter region showed methylation in 25 out of 31 tumours (80%) and all four normal tissues (P=NS C- square test). A trend towards an association was seen between miR-9 methylation status and patient¡¯s age, and Ki67 expression (Mann Whitney U Test). Our results suggest a possible role of miR9 methylation and breast cancer carcinogenesis. We are currently validating these results on an extended data sample of 72 breast cancer cases.
%U http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/947