%0 Journal Article
%T Experimental evidence of a xylose-catabolic pathway on the pAO1 megaplasmid of Arthrobacter nicotinovorans
%A Marius Mihasan
%A Marius Stefan
%A Vlad Artenie
%J Analele £¿tiin£¿ifice Ale Universit£¿£¿ii Alexandru Ioan Cuza din Ia£¿i,Sectiunea II A : Genetica si Biologie Moleculara
%D 2012
%I Alexandru Ioan Cuza University of Iasi
%X The pAO1 megaplasmid of A. nicotinovorans consists of 165 ORF's related mainly to nicotine degradation, uptake and utilization of carbohydrates, amino acids and sarcosine. A putative sugar catabolic pathway consisting of 11 ORF's organized as a single operon were previously described. The current work brings experimental data supporting the existence of a D-Xylose catabolic pathway on the pAO1 megaplasmid. When grown on D-xylose containing media, the cells harboring the pAO1 megaplasmid grow to higher cell densities and also express the OxRe protein coded by the megaplasmid. A putative pathway similar to Weimberg pentose pathway is postulated, in which D-xylose is transported in the cell by the ABC-type transport system and then transformed using the putative sugar-dehidrogenase OxRe to D-xylonate, which is further degrated to 2-ketoglutarate and integrated into the general metabolism of the cell
%K Arthrobacter
%K xylose
%K pAO1
%U http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/264