%0 Journal Article
%T Immunophenotype and differentiation capacity of bone marrow-derived mesenchymal stem cells from CBA/Ca, ICR and Balb/c mice
%A Yin Yin Ooi
%A Zul¡¯atfi Rahmat
%A Shinsmon Jose
%A Rajesh Ramasamy
%J World Journal of Stem Cells
%D 2013
%I Baishideng Publishing Group Co. Limited
%R 10.4252/wjsc.v5.i1.34
%X AIM: To assess the capacity to isolate and expand mesenchymal stem cells (MSC) from bone marrow of CBA/Ca, ICR and Balb/c mice. METHODS: Bone marrow of tibia and femur were flushed, cultured and maintained in supplemented Dulbecco¡¯s modified Eagle¡¯s medium. MSC immunophenotype of cultures were tracked along increasing passages for positivity to CD106, Sca-1 and CD44 and negativity to CD45, CD11b and MHC class II. Differentiation capacity of MSC towards osteogenic and adipogenic lineages were also assessed. RESULTS: MSC were successfully cultured from bone marrow of all 3 strains, albeit differences in the temporal expression of certain surface antigens. Their differentiation into osteocytes and adipocytes were also observed. MSC from all 3 mouse strains demonstrated a shift from a haematopoietic phenotype (CD106-CD45+CD11b+Sca-1low) to typical MSC phenotype (CD106+CD45-CD11b-Sca-1high) with increasing passages. CONCLUSION: Information garnered assists us in the decision of selecting a mouse strain to generate MSC from for downstream experimentation.
%K Mesenchymal stem cells
%K Mouse bone marrow
%K CBA/Ca strain
%K ICR strain
%K Balb/c strain
%K Immunophenotyping
%K Differentiation
%U http://www.wjgnet.com/1948-0210/full/v5/i1/34.htm