%0 Journal Article
%T Non-random tissue distribution of human na ve umbilical cord matrix stem cells
%A Dharmendra Kumar Maurya
%A Chiyo Doi
%A Marla Pyle
%A Raja Shekar Rachakatla
%J World Journal of Stem Cells
%D 2011
%I Baishideng Publishing Group Co. Limited
%R 10.4252/wjsc.v3.i4.34
%X AIM: To determine the tissue and temporal distribution of human umbilical cord matrix stem (hUCMS) cells in severe combined immunodeficiency (SCID) mice. METHODS: For studying the localization of hUCMS cells, tritiated-thymidine labeled hUCMS cells were injected in SCID mice and tissue distribution was quantitatively determined using a liquid scintilation counter at days 1, 3, 7 and 14. Furthermore, an immunofluorescence detection technique was employed in which anti-human mitochondrial antibody was used to identify hUCMS cells in mouse tissues. In order to visualize the distribution of transplanted hUCMS cells in H&E stained tissue sections, India Black ink 4415 was used to label the hUCMS cells. RESULTS: When tritiated thymidine-labeled hUCMS cells were injected systemically (iv) in female SCID mice, the lung was the major site of accumulation at 24 h after transplantation. With time, the cells migrated to other tissues, and on day three the spleen, stomach and small and large intestine were the major accumulation sites. On day seven, a relatively large amount of radioactivity was detected in the adrenal gland, uterus, spleen, lung, and digestive tract. In addition, labeled cells had crossed the blood brain barrier by day 1. CONCLUSION: These results indicate that peripherally injected hUCMS cells distribute quantitatively in a tissue-specific manner throughout the body.
%K Human umbilical cord matrix stem cells
%K Immunohistochemistry
%K India black ink loaded cells
%K Radio-labeled transplanted cells
%K Tissue distribution
%U http://www.wjgnet.com/1948-0210/full/v3/i4/34.htm