%0 Journal Article
%T Simultaneous quantification of zidovudine, stavudine, lamivudine and nevirapine by Micellar Electrokinetic Capillary Chromatography Quantification simultan¨¦e de zidovudine, stavudine, lamivudine et n¨¦virapine par chromatographie ¨¦lectrocin¨¦tique micellaire
%A Gras Alain
%A Yegles Michel
%A Karasi Jean-Claude
%A Schmit Jean-Claude
%J Annales de Toxicologie Analytique
%D 2013
%I 
%R 10.1051/ata/2012024
%X Purpose: Recent improvements to the availability of antiretroviral therapy in Sub-Saharan Africa can be attributed to the generic formulation of antiretroviral drugs. Quality drug surveillance, routine supervision and adherence data are however restricted owing to a fundamental lack of resources in the area. Accordingly we have developed an affordable micellar electrokinetic capillary chromatography (MEKC) method for the simultaneous detection and quantification of zidovudine, stavudine, lamivudine and nevirapine in plasma. Methods: The antiretroviral drugs were extracted by solid phase extraction. Various factors influencing separation of the four drugs have been optimized. A buffer consisting of 5 mM sodium tetraborate at pH 9.8, containing 50 mM SDS, 30% methanol and 5% ethanol was found to be particularly suitable and the MEKC method was validated. Results: All validation parameters were within the 20% acceptation limit, except for the interday precision of stavudine which required a daily calibration curve. The limit of quantification (LOQ) for zidovudine, stavudine, lamivudine and nevirapine were 0.037, 0.051, 0.029 and 0.028 mg/L respectively and were below the therapeutic concentration ranges of each drug. The optimized MEKC method was successfully applied to 16 human plasma samples. Conclusion: Our sensitive and validated method was demonstrated to be suitable for simultaneous detection and quantification of zidovudine, stavudine, lamivudine and nevirapine. This cost-effective method could be of interest for resource limited countries not only for adherence or therapeutic monitoring but also for steady-state pharmacokinetic studies of generic ARV drugs. Objectif : L¡¯acc¨¨s aux th¨¦rapies antir¨¦trovirales en Afrique sub-saharienne s¡¯est r¨¦cemment nettement am¨¦lior¨¦ grace ¨¤ l¡¯apparition des antir¨¦troviraux sous la forme de g¨¦n¨¦riques. Cependant, la surveillance de la qualit¨¦ des m¨¦dicaments, le suivi th¨¦rapeutique de routine et les donn¨¦es concernant l¡¯observance restent limit¨¦s dans ces r¨¦gions du fait du manque de ressources. Par cons¨¦quent, nous avons d¨¦velopp¨¦ une m¨¦thode de chromatographie ¨¦lectrocin¨¦tique micellaire (CEM) ¨¤ faible co t permettant la d¨¦tection et la quantification simultan¨¦e de zidovudine, stavudine, lamivudine et n¨¦virapine dans le plasma. M¨¦thodes : Une extraction en phase solide a ¨¦t¨¦ r¨¦alis¨¦e afin d¡¯isoler les m¨¦dicaments antir¨¦troviraux. Diff¨¦rents facteurs influen ant la s¨¦paration des quatre composants ont ¨¦t¨¦ optimis¨¦s. Un tampon constitu¨¦ de t¨¦traborate de sodium 5 mM ¨¤ pH 9,8, contenant 50 mM de SDS, 30 % m¨¦thanol et
%K Capillary electrophoresis
%K drug monitoring
%K highly active antiretroviral therapy
%K Micellar Electrokinetic Capillary Chromatography
%K plasma
%K ¨¦lectrophor¨¨se capillaire
%K pharmacovigilance
%K traitement antir¨¦troviral hautement actif
%K chromatographie ¨¦lectrocin¨¦tique micellaire
%K plasma sanguin
%U http://dx.doi.org/10.1051/ata/2012024