%0 Journal Article
%T Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Orthogonal Fluorescent Compound
%A Jae-Jung Lee
%A Jun-Young Kim
%A Duanting Zhai
%A Seong-Wook Yun
%J Interdisciplinary Bio Central
%D 2012
%I IBC
%X Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose ˇ°Estern stainingˇ±, as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming ˇ°immuno-stainingˇ± in many versatile applications.
%K Western blot
%K Estern staining
%K fluorescence dye BDED
%K RC-tag
%K peptide chemical tags
%U http://www.ibc7.org/article/journal_v.php?sid=281&page=1