%0 Journal Article
%T Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles
%A Daniel G Olson
%A Lee R Lynd
%J Journal of Biological Engineering
%D 2012
%I BioMed Central
%R 10.1186/1754-1611-6-5
%X A library of 34 mutant plasmids was designed, synthesized and screened, resulting in 6 mutants which exhibited a Ts phenotype. Of these 6, the one with the most temperature-sensitive phenotype (M166A) was compared with the original plasmid. It exhibited lower stability at 48ˇăC and was completely unable to replicate at 55ˇăC.The plasmid described in this work could be useful in future efforts to genetically engineer C. thermocellum, and the method used to generate this plasmid may be useful for others trying to make Ts plasmids.
%K Temperature sensitive
%K Rolling circle
%K Plasmid
%K Clostridium thermocellum
%K Gram positive
%K Thermophile
%U http://www.jbioleng.org/content/6/1/5/abstract