%0 Journal Article
%T Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats
%A Pei-Jiun Tsai
%A Hwai-Shi Wang
%A Yi-Ming Shyr
%A Zen-Chung Weng
%A Ling-Chen Tai
%A Jia-Fwu Shyu
%A Tien-Hua Chen
%J Journal of Biomedical Science
%D 2012
%I BioMed Central
%R 10.1186/1423-0127-19-47
%X MSCs from human umbilical cord were induced in three stages to differentiate into insulin-producing cells and evaluated by immunocytochemistry, reverse transcriptase, and real-time PCR, and ELISA. Differentiated cells were transplanted into the liver of diabetic rats using a Port-A catheter via the portal vein. Blood glucose levels were monitored weekly.Human nuclei and C-peptide were detected in the rat liver by immunohistochemistry. Pancreatic ¦Â-cell development-related genes were expressed in the differentiated cells. C-peptide release was increased after glucose challenge in vitro. Furthermore, after transplantation of differentiated cells into the diabetic rats, blood sugar level decreased. Insulin-producing cells containing human C-peptide and human nuclei were located in the liver.Thus, a Port-A catheter can be used to transplant differentiated insulin-producing cells from human MSCs into the portal vein to alleviate hyperglycemia among diabetic rats.
%K Mesenchymal stem cell
%K Portal vein
%K Insulin-producing cells
%K Transplant
%U http://www.jbiomedsci.com/content/19/1/47/abstract