%0 Journal Article
%T Abstract
%A Celina Alcoforado Santos
%A Dominik Lenz
%A Geisamanda Pedrini Brandˋo
%A Adriana Regina Chippari-Gomes
%J Neotropical Ichthyology
%D 2013
%I Sociedade Brasileira de Ictiologia
%X Diesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboides were assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure. O 車leo diesel pode ser uma fonte de contamina  o em ambientes aqu芍ticos, principalmente como resultado de derrames acidentais. Foram avaliados os efeitos da fra  o sol迆vel do 車leo diesel (FSO) em Prochilodus vimboides. Os peixes foram expostos a tr那s diferentes dilui  es da FSO por at谷 96 horas e comparados com um grupo controle. Os danos nos fragmentos de DNA foram analisados utilizando o ensaio Cometa. A presen a de anormalidades nos eritr車citos foi avaliada pelo teste do micron迆cleo. A atividade da Aspartato aminotransferase (AST) e alanina aminotransferase (ALT) e da acumula  o de cobre nas branquias tamb谷m foram analisados. Os peixes expostos por 96 h tiveram maiores taxas de danos do que aqueles expostos por 24 h. N o houve diferen a significativa quanto 角 presen a de micron迆cleos entre os peixes expostos e controle e entre 24 e 96 h exposi  es. Para AST, n o foi observada diferen a significativa entre as amostras coletadas em dois tempos de exposi  o. Os peixes expostos a uma dilui  o de 1:100 do FSO apresentaram maior atividade da enzima ALT do que os peixes do controle ap車s um per赤odo de exposi  o de 24 horas. N o houve bioacumula  o de cobre nas branquias. Os efeitos genot車xicos nas c谷lulas foram os mais evidentes em P. vimboides durante a exposi  o aguda a FSO.
%K Comet assay
%K Curimba
%K Diesel
%K Micronucleus
%U http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193